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KMID : 0382619870070010363
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Hanyang Journal of Medicine
1987 Volume.7 No. 1 p.363 ~ p.376
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Isolation and Properties of Neutral Ribonucleases in Serum of Patients with Lymphocytic Leukemia
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Abstract
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Activities of neutral RNase were measured in serum of both normal control and
01 acute lymphocytic leukemia to find out whether the serum enzyme could be used as a diagnostic marker for the leukemia. Employing DEAE-cellulose column chromatography and PAGE, neutral RNases and proteins were isolated and fractionated from serum of both normal control and acute lymphocytic leukemia. The enzymes thus isolated were used for study of specific activity, substrate specificity and degradation process of the substrate.
i 1) The activity of neutral RNase was increased significantly in serum of acute lymphocytic leukemia by 48% over normal control. The positive rate of the enzyme as a marker for the leukemia was found to be 40%.
2) DEAE-cellulose column chromatographical analyses for serum RNases and proteins revealed that serum RNases were separated into 4 peaks each for normal control and acute lymphocytic leukemia and serum proteins were fractionated into 8 peaks for normal control and 7 peaks for the leukemia. The peak IVb and VI proteins present in normal serum were not found in the leukemic serum, and the peak V present in the leukemic serum was absent in normal serum.
3) The specific activity of DEAE-cellulose peak III neutral RNase was great-
´r ly increased in acute lymphocytic leukemia, and the substrate specificity for each of four peak neutral RNases in the leukemic serum appeared to be different from that in normal serum.
rill 4) The chromatographical pattern of poly C digest by peak III neutral RNase
from the leukemic serum appeared to be different from that of normal serum.
5) A study for separation of normal and leukemic serum proteins on native PAGE indicated that two protein bands were suppressed and 4 protein bands absent in normal serum were observed in serum of acute lymphocytic leukemia.
These results suggested that the peak III neutral RNases isolated from serum of acute lymphocytic leukemia might be the enzyme specific to the leukemia and that at least two protein bands were suppressed and more than 4 protein bands were found to be specific for the leukemia.
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KEYWORD
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